Fluorescent micrograph of a blastomere a single cell from a developing embryo stained to detect chromosome abnormalities. A similar MTT assay has been developed for testing the viability of sperm of fowls Hazary et al. In most cases the location of the sperm head granule was near the ridge of the acrosomal cap. Finally, a technique preferred by many for evaluating sperm morphology is to use no stain at all, but to visualize sperm under differential interference contrast microscopy. Place the edge of another slide into the drops of stain and semen - rock that slide back and forth a few times to mix the sperm and stain, then smear the second slide across the surface of the first. The author thanks Prof.
The MTT assay for sperm has been claimed to be a reliable test method and practical for routine evaluation of semen Hazary et al.
Microscopic analysis of MTT stained boar sperm cells
Carmichael J, DeGraf W. SSIM index for all pixels are calculated and scaled [0,]these values are shown in Figure 6 d. The increase in size reaches a maximum at about 1 to 2 h of staining, while the multiple granules became more distinct in the midpiece. The evaluation is summarized in Table 1. Flourescent micrograph of sperm stained with propidium iodide, red pisum sativum agglutinin PSAgreen.